Blood Plasma Biochemical Changes and Conception Rate in Anoestrus Buffaloes Treated with Various Hormone Protocols

To study certain blood biochemical parameters (total cholesterol, total protein, calcium and inorganic phosphorus) at various periods of treatment. Total 40 anestrous buffalo heifers with history of anoestrus were selected from the semi-arid area of Banaskanth and randomly divided into four equal groups. (1)Group-I:Heatsynch protocol (n=10), (2)Group-II:Ovsynch protocol(n=10), (3)Group-III: Doublesynch protocol (n=10) and (4)Group-IV: Control(n=10) followed by fixed time artificial insemination in treated buffalo heifers and insemination at spontaneous estrus in buffalo heifers of group-IV. The overall conception rate following 3 consecution services was maximum in Group II (50%) followed by Group III (40%), Group I (30%) and Group IV (30%). The blood plasma was collected at the time of initiation of treatment (P1), at the time of inj. PGF2α (P2), at the time of 1st FTAI (P3) and day 21st post insemination (P4) for the estimation of biochemical parameters. The blood biochemical profiles include total protein (range: 4.54± 0.19 to 6.11± 0.52 g/dl) which in all group did not show any significant difference during the different period but significant (P<0.05) difference between the groups was noticed. Similarly calcium and phosphorus found ranging between 5.73± 0.20 to 9.53± 0.95 mg/dl and 5.46± 0.22 to 6.95± 0.30 mg/dl, respectively in all four groups. The cholesterol estimated values (range: 34.12 ± 1.75 to 41.97± 2.43 mg/dl) were found not-significantly varying between the groups as well as among the different periods. Heatsynch, Ovsynch and Doublesynch protocol can be successfully used for the induction of estrus in true anoestrus buffalo heifers with Better conception rate would be achieved following Ovsynch protocol in buffalo heifers.