Morazzoni, Paolo and Vanzani, Paola and Santinello, Sandro and Gucciardi, Antonina and Zennaro, Lucio and Miotto, Giovanni and Ursini, Fulvio (2021) Grape Seeds Proanthocyanidins: Advanced Technological Preparation and Analytical Characterization. Antioxidants, 10 (3). p. 418. ISSN 2076-3921
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Abstract
A “green” solvent-free industrial process (patent pending) is here described for a grape seed extract (GSE) preparation (Ecovitis™) obtained from selected seeds of Veneto region wineries, in the northeast of Italy, by water and selective tangential flow filtration at different porosity. Since a comprehensive, non-ambiguous characterization of GSE is still a difficult task, we resorted to using an integrated combination of gel permeation chromatography (GPC) and electrospray ionization high resolution mass spectrometry (ESI-HRMS). By calibration of retention time and spectroscopic quantification of catechin as chromophore, we succeeded in quantifying GPC polymers up to traces at n = 30. The MS analysis carried out by the ESI-HRMS method by direct-infusion allows the detection of more than 70 species, at different polymerization and galloylation, up to n = 13. This sensitivity took advantage of the nanoscale shotgun approach, although paying the limit of missed separation of stereoisomers. GPC and MS approaches were remarkably well cross-validated by overlapping results. This simple integrated analytical approach has been used for quality control of the production of Ecovitis™. The emerging feature of Ecovitis™ vs. a popular benchmark in the market, produced by a different technology, is the much lower content of species at low n and the corresponding increase of species at high n.
Item Type: | Article |
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Subjects: | Eprints AP open Archive > Agricultural and Food Science |
Depositing User: | Unnamed user with email admin@eprints.apopenarchive.com |
Date Deposited: | 07 Jul 2023 04:47 |
Last Modified: | 22 Nov 2023 05:39 |
URI: | http://asian.go4sending.com/id/eprint/869 |