OPTIMIZATION OF CULTURE CONDITIONS FOR AMYLASE PRODUCTION FROM LOCALLY ISOLATED Aspergillus sp.

In the present study, two locally isolated fungal strains (Aspergillus sp. 1 and Aspergillus sp. 2) were used for amylase production under submerged fermentation. Before optimization, Aspergillus sp. 1 and 2 demonstrated maximum amylase production of 82 ± 0.79 IU/mL and 110 ± 0.32 IU/mL, respectively. One variable at a time approach was used for optimization of culture conditions such as carbon source, nitrogen source, pH and incubation time. Optimized parameters for increased amylase production from Aspergillus sp. 1 was found to at pH 10.0 after 4 days of incubation using fructose as carbon source and ammonium sulfate as nitrogen source. Similarly, Aspergillus sp. 2 demonstrated increased amylase production at pH 10.0 of production medium after 4 days of incubation using fructose as carbon source and peptone as nitrogen source. After single parameter optimization amylase activity from Aspergillus sp. 1 and Aspergillus sp. 2 was found to be 161.23 ± 1.28 IU/mL and 166.73 ± 1.12 IU/mL, respectively. Further, response surface methodology (RSM) based central composite design (CCD) was used for optimization of amylase production from Aspergillus sp. 2. Maximum amylase production (172.36 IU/mL) was found using 1% sucrose concentration, 1.05% peptone concentration at pH 11 after 5 days of incubation time.