Protective Effects of Vitamins C and E on Dimethoate- Induced Nephrotoxicity in Male Guinea Pigs

Aims: To examine the protective properties of vitamin C (vitC) and E (vitE) on DM induced nephrotoxicity in male guinea pigs.
Study Design: Twenty male rats were randomly grouped into four. “1” = Control, “2” = Vitamins (C & E) treated, “3” = Dimethoate (DM) treated and “4” = Vitamins treated plus DM treated.
Place and Duration of Study: Department of Biology, Ibb University, Ibb, Yemen between February 2011 and May 2011.
Methodology: Four animal groups were used, G1: received 1.0ml of distilled water and 1.0ml of olive oil, G2: received 200mg/kg b.w/day of vitamins (C & E), G3: was treated with 7mg/kg b.w/day DM and G4: was treated with 200mg/kg b.w/day of vitamins (C & E) plus 7mg/kg b.w/day DM. All the previous administrations were repeated daily for 28 days. At the end of the experiment, the animals were sacrificed and dissected. Blood and tissue samples were taken for biochemical, and light microscope investigations.
Results: DM administration resulted in a significant increase in the serum urea and creatinine levels and lipid peroxidation (LPO) level, while it caused significant decreases in the activities of catalase (CAT) and glutathione-S-transferase (GST). In contrast, co-administration of vitC and vitE to DM-treated animals restored most of these biochemical parameters to nearly normal levels. Also, DM induced histopathological changes in the kidneys of all treatment groups but these alterations were predominant in DM-treated group.
Conclusion: The results showed that co-treatment of vitE and vitC may protect the guinea pigs from DM-induced nephrotoxicity.