Study of Genetic Diversity among Different Tomato (Lycopersicum esculentum Mill.) Lines through Morphological and Biochemical Analysis

Aims: The aims of this study were to characterize the existing genetic diversity from Azad Jammu & Kashmir and to identify some selectable markers based on total leaf protein profiles. The purpose of the investigation was to detect any possible variations among selected cultivars and to document the protein profiling for future record. The total leaf proteins profile based on SDS-PAGE showed bands of lower molecular weight common among all the varieties.

Study Design: Study was conducted on the basis of randomized complete block design.

Place and Duration of Study: Sample: Field experiment was conducted in different plots in all three districts Mirpur, Bhimber and Kotli and lab experiment was conducted in Department of Botany Mirpur University of Science and Technology during the year 2012-2014.

Methodology: Please write main points of the research methodology applied. Plant sample such as 1 Red wonder 2 Tomato Galia 3 Rizwan tomato Super special -F1 4 Tomato Red King -F1 5 Tomato 117-F1 collected and Following parameters were studied Morphological analys Biochemical analysis by SDS PAGE and Statistical analysis was performed.

Results: Results was confirmed through cluster analysis, principal component analysis and Minitab. Dendrogram was achieved from the cluster analysis of five varieties of Lycopersicum esculentum was based on five morphological traits. Distance estimated based on five morphological traits ranged from 6-60 with an average of 33 Proteins distribution patterns of fourteen cultivars of Lycopersicum esculentum disclose there are significant variations in terms of banding pattern. Analysis of gel electrophorogram of SDS PAGE showed that there are significant differences between the bands of all fourteen cultivars. There were total fourteen bands present in each tomato variety the molecular weight of marker ranges between 10-200 kDa. There were two bands No. 12 and 14 common among all fourteen cultivars. These bands are of low molecular weight with the molecular weight of 20 and 10 kDa, respectively.

Conclusion: It is concluded from present work that genetic diversity existed among all the cultivars. On the basis of all parameter studied, it was found that Red wonder is the best among all lines.